Nrg1^tm1Fej
Targeted Allele Detail

Summary

• Symbol: Nrg1^tm1Fej
• Name: neuregulin 1; targeted mutation 1, Frank E Jones
• MGI ID: MGI:2448075
• Synonyms: HRGalpha-null
• Gene: Nrg1 Location: Chr8:32299493-33381858 bp, - strand Genetic Position: Chr8, 18.75 cM
• Alliance: Nrg1^tm1Fej page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81107
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The endogenous locus was modified to contain a stop codon in exon 7 and single loxP sites flanking exons 7, 8, and 9. The mutation was designed to specifically target the alpha isoform, which contains exon 7. RT-PCR analysis showed reduced levels of mRNA, putatively due to nonsense mediated decay. (J:81107)

Expression

In Mice Carrying this Mutation:

104036 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nrg1 Mutation: 53 strains or lines available

References

• Original: J:81107 Li L, et al., The breast proto-oncogene, HRGalpha regulates epithelial proliferation and lobuloalveolar development in the mouse mammary gland. Oncogene. 2002 Jul 25;21(32):4900-7
• All: 5 reference(s)