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Targeted Allele Detail
Symbol: Apoetm1Khw
Name: apolipoprotein E; targeted mutation 1, Karl H Weisgraber
MGI ID: MGI:2447429
Synonyms: Apoeh, ApoeR61h, ApoeRh, Arg-61neo+
Gene: Apoe  Location: Chr7:19696109-19699188 bp, - strand  Genetic Position: Chr7, 9.94 cM
Germline Transmission:  Earliest citation of germline transmission: J:75681
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Humanized sequence, Hypomorph)
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsA threonine to arginine substitution was introduced into amino acid 61 (T61R) of mouse Apoe exon 3 and a floxed neo cassette was inserted into the downstream intron via homologous recombination. This mutation allows the mouse protein to mimic human APOE4 domain interaction. The presence of the neo cassette renders this allele hypomorphic with mRNA expression at 2%-5% that of wild-type. Expression of the mutant allele was observed by Western blot analysis. (J:75681)
View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Apoe Mutation:  68 strains or lines available
Original:  J:75681 Raffai RL, et al., Hypomorphic Apolipoprotein E Mice. A NEW MODEL OF CONDITIONAL GENE REPAIR TO EXAMINE APOLIPOPROTEIN E-MEDIATED METABOLISM. J Biol Chem. 2002 Mar 29;277(13):11064-8
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.04
The Jackson Laboratory