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Targeted Allele Detail
Symbol: Cd247/Pou2f1tm1Tks
Name: POU domain, class 2, transcription factor 1; targeted mutation 1, Takashi Saito
MGI ID: MGI:2447062
Synonyms: Cd247tm1Tks, Cd247tm2Tks, Cd3z/Pou2f1tm1Tks, etaT
Gene: Pou2f1  Location: Chr1:165865154-166002678 bp, - strand  Genetic Position: Chr1, 73.21 cM
Germline Transmission:  Earliest citation of germline transmission: J:17251
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intergenic deletion
Mutation detailsThe replacement of a 1.4 kb genomic fragment with a neo cassette resulted in the disruption of both the Cd247 and Pou2f1 loci, which are located at the same chromosomal position on opposite strands. The deletion of a 3' portion of Cd247 exon 9 and its splice acceptor site disrupted sequence encoding the eta isoform while leaving the sequence encoding the zeta isoform intact. Neither eta transcript nor protein was detected in homozygous mutant mice while normal expression of zeta chain homodimers was confirmed by 2D SDS-PAGE analysis. The insertion of the neo cassette also replaced the 3' UTR of the Pou2f1 locus, resulting in reduced expression of normal transcript as well as in an induced expression of aberrant transcript. (J:17251)
View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pou2f1 Mutation:  74 strains or lines available
Original:  J:17251 Ohno H, et al., Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand. EMBO J. 1994 Mar 1;13(5):1157-65
All:  1 reference(s)

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MGI 6.05
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