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Targeted Allele Detail
Symbol: Cd4tm2Litt
Name: CD4 antigen; targeted mutation 2, Dan R Littman
MGI ID: MGI:2446611
Synonyms: CD4S4delta, Cd4sil-
Gene: Cd4  Location: Chr6:124864692-124888221 bp, - strand  Genetic Position: Chr6, 59.17 cM
Germline Transmission:  Earliest citation of germline transmission: J:80576
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Modified regulatory region, Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsThe core region of a cis regulatory element that silences expression of the endogenous locus during T cell development was deleted from intron 1 via homologous recombination of a targeting construct lacking nucleotides 167 through 257. A single loxP site was left upstream of the deleted region, however analysis of a control allele indicated that the loxP site did not affect normal expression. Flow cytometric analysis of peripheral blood lymphocytes obtained from mutant mice showed no silencing of Cd4 expression. (J:80576)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd4 Mutation:  65 strains or lines available
Additional alleles engineered to lack nucleotides within the core silencer and others engineered to lack surrounding sequences showed variegated expression of Cd4.
Original:  J:80576 Taniuchi I, et al., Evidence for distinct CD4 silencer functions at different stages of thymocyte differentiation. Mol Cell. 2002 Nov;10(5):1083-96
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory