Ebf2^tm1.1Ggc
Targeted Allele Detail

Summary

• Symbol: Ebf2^tm1.1Ggc
• Name: early B cell factor 2; targeted mutation 1.1, Giacomo Consalez
• MGI ID: MGI:2446576
• Synonyms: Ebf2-lacZ
• Gene: Ebf2 Location: Chr14:67470741-67668367 bp, + strand Genetic Position: Chr14, 34.63 cM, cytoband D-E1
• Alliance: Ebf2^tm1.1Ggc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80220
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was removed from Ebf2^tm1Ggc via in vivo cre mediated recombination. The lacZ gene remained in frame and in place of the 5.2 kb fragment containing the coding regions of the first five exons. The deleted region included sequence encoding the majority of the DNA binding domain. The absence of full length transcript was confirmed by RT-PCR analysis of homozygous mutant mice. lacZ expression was determined to be driven by the endogenous promoter. (J:80220)

Expression

In Mice Carrying this Mutation:	125 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ebf2 Mutation: 30 strains or lines available

References

• Original: J:80220 Corradi A, et al., Hypogonadotropic hypogonadism and peripheral neuropathy in Ebf2-null mice. Development. 2003 Jan;130(2):401-10
• All: 11 reference(s)