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Targeted Allele Detail
Symbol: Ltb/Tnftm1.1Dvk
Name: tumor necrosis factor; targeted mutation 1.1, Dmitry V Kuprash
MGI ID: MGI:2446565
Synonyms: (LTBeta/TNF)delta
Gene: Tnf  Location: Chr17:35199381-35202007 bp, - strand  Genetic Position: Chr17, 18.59 cM
Germline Transmission:  Earliest citation of germline transmission: J:58981
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Intergenic deletion
Mutation detailsThe floxed 5.3 kb region, containing exon 3 of the Ltb locus and exons 3 and 4 of the Tnf locus, was excised via in vivo cre mediated recombination between the loxP site within the Ltb locus and the loxP site within the Tnf locus. The deleted regions encoded the majority of the extracellular region of Ltb and the entire secreted portion of Tnf. An RNAse protection assay using homozygous mutant concavalin A stimulated splenocytes detected a truncated Ltb transcript and demonstrated normal transcription of the closely linked Lta locus. (J:58981)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tnf Mutation:  35 strains or lines available
Original:  J:58981 Kuprash DV, et al., TNF and lymphotoxin beta cooperate in the maintenance of secondary lymphoid tissue microarchitecture but not in the development of lymph nodes. J Immunol. 1999 Dec 15;163(12):6575-80
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
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