Nr4a2^tm1Niko
Targeted Allele Detail

Summary

• Symbol: Nr4a2^tm1Niko
• Name: nuclear receptor subfamily 4, group A, member 2; targeted mutation 1, Vera M Nikodem
• MGI ID: MGI:2429971
• Synonyms: Nurr1
• Gene: Nr4a2 Location: Chr2:56996845-57014018 bp, - strand Genetic Position: Chr2, 31.66 cM
• Alliance: Nr4a2^tm1Niko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47684
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded amino acids 1 through 288, encompassing the initiating methionine and the first zinc finger domain. Transcript lacking exon 3 was detected in mutant mice by Northern blot analysis of brain tissue. Normal transcript was undetected in the substantia nigra and ventral tegmental area by in situ hybridization. (J:47684)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nr4a2 Mutation: 40 strains or lines available

References

• Original: J:47684 Castillo SO, et al., Dopamine biosynthesis is selectively abolished in substantia nigra/ventral tegmental area but not in hypothalamic neurons in mice with targeted disruption of the Nurr1 gene. Mol Cell Neurosci. 1998 May;11(1-2):36-46
• All: 10 reference(s)