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Raf1tm1Zim
Targeted Allele Detail
Nomenclature
Symbol: Raf1tm1Zim
Name: v-raf-leukemia viral oncogene 1; targeted mutation 1, Andreas Zimmer
MGI ID: MGI:2429683
Synonyms: Craf-1neo2
Gene: Raf1  Location: Chr6:115618067-115676635 bp, - strand  Genetic Position: Chr6, 53.62 cM, cytoband C3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:50696
Parent Cell Line:  MPI-II (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Hypomorph)
Mutation:    Insertion
 
Mutation detailsA hypomorphic allele was generated by disrupting the first coding exon (exon 2) with a neo cassette. While Western blot analysis showed an absence of normal protein (74 kDA), low levels of a 62 kDa protein were identified. Sequence analysis of mRNA showed excision of the targeted exon and identified potential start codons in exon 3. Biochemical assays showed no kinase activity in nonstimulated mutant fibroblasts and a 90% reduction in activity in stimulated fibroblasts relative to wild-type samples. (J:50696)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Raf1 Mutation:  95 strains or lines available
References
Original:  J:50696 Wojnowski L, et al., Craf-1 protein kinase is essential for mouse development. Mech Dev. 1998 Aug;76(1-2):141-9
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory