About   Help   FAQ
Mobq4C57BL/6J
QTL Variant Detail
Nomenclature
QTL variant: Mobq4C57BL/6J
Name: multigenic obesity QTL 4; C57BL/6J
MGI ID: MGI:2429524
QTL: Mobq4  Location: Chr15:3460212-3460347 bp  Genetic Position: Chr15, cM position of peak correlated region/allele: 1.84 cM
QTL Note: genome coordinates based on the marker associated with the peak LOD score
Variant
origin
Strain of Specimen:  C57BL/6J
Variant
description
Allele Type:    QTL
Inheritance:    Recessive
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Notes

Candidate Genes

J:91835

The correlation between gene expression and dietary interaction was examined in a population of (BALB/cStCrlfC3H/Nctr x VY/WffC3Hf/Nctr-A(vy)/a)F1 animals. A/a and A/Avy animals were placed on a 70% (calorie restricted) or 100% (non-restricted) diet andliver mRNA levels were assessed for over 18,000 genes using DNA microarrays. Twenty-eight known genes showing statistically significant differential expression between the 70% and 100% calorie diets and A/a and A/Avy genotypes mapped near known diabesity QTLs. These genes may be considered further for candidate genes.

On mouse Chromosome 15, Npr3 (6.7 cM) shows differential expression between restricted and unrestricted diets on the A/Avy background as well as between A/a and A/Avy animals on the restricted diet. Diabesity QTLs Bw11 (6 cM), Mob4 (6.7 cM), and Dob9 (12.3 cM) map near Npr3. Candidate genes for diabesity QTLs Dbsty4 (49.6 cM) and Bwtq6 (41.2 cM) are D15Wsu75e (46.7 cM), Gpt1 (40.3 cM), Tef (46.7 cM), and H1f0 (46.75 cM). Tef and H1f0both show differential expression between restricted and unrestricted diets on the A/Avy background. D15Wsu75e shows differential expression between A/a and A/Avy animals on both the restricted and unrestricted diets. Gpt1 shows differential expression between A/a and A/Avy animals on the restricted diet.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:24238

Mob1 and Mob2, two previously identified QTLs affecting obesity on mouse Chromosome 7 and mouse Chromosome 6, respectively, were confirmed in a (C57BL/6J x SPRET/Ei)F1 x C57BL/6J backcross population. Interestingly, parental strains C57BL/6J and SPRET/Eiare both lean.

In the present study Mob1 gave a LOD score of 4.6 at D7Mit8 in association with percent body water, and is significantly linked to hepatic lipase activity (LOD = 5.1). Mob1 also showed linkage to total plasma cholesterol (LOD = 4.4), bodymassindex (LOD = 2.9), body weight (LOD = 2.9), and HDL cholesterol level (LOD = 3.1). Animals heterozygous at Mob1 exhibit 25% increase in body fat compared to animals homozygous for C57BL/6J-derived alleles at Mob1. Congenic strains B10.129(5M)/nSN andB10.C(41N)/Sn carry 129/J-derived and BALB/cJ-derived alleles at Mob1, respectively, on a C57BL/10SnJ background. Animals from both congenic lines exhibit decreased fat pad weight, body weight, and plasma cholesterol compared to C57BL/10SnJ. B10.129(5M)/nSN also exhibits decrease in hepatic lipase activity. Possible candidate genes in the region of Mob1 are Snrpn, Tub, Ath3, Igf1r, Ins2 and Sult1a1.

Mob2 gave a peak LOD score of 4.8 at D6Mit1 in association with femoral fat pad weight. Mob2 also showed linkage to total plasma cholesterol (LOD = 3.4) and percent body water (LOD = 3.2). Animals heterozygous at Mob2 exhibit a 2-fold increase in femoral fat pad weight compared to animals homozygous for C57BL/6J-derived alleles at Mob2. A possible candidate gene for Mob2 is Lep (ob).

Two novel QTLs, Mob3 and Mob4, mapped to mouse Chromosome 12 and mouse Chromosome 15, respectively. Mob3 is associated with percent body fat with a LOD score of 4.8 at D12Mit27 on mouse Chromosome 12. Heterozygosityat Mob3 results in 44% increase in body fat compared to animals homozygous for C57BL/6J-derived alleles at Mob3. A possible candidate gene for Mob3 is Tshr. Mob4 is associated with mesenteric fat pad weight with a LOD score of 3.4 at D15Mit13 on mouse Chromosome 15. Animals heterozygous at Mob4 exhibit 32% decrease in mesenteric fat pad weight compared to animals homozygous for C57BL/6J-derived alleles at Mob4. A possible candidate gene for Mob4 is Ghr. Mob1, Mob2, Mob3, and Mob4 do not appear to interact.

References
Original:  J:24238 Warden CH, et al., Identification of four chromosomal loci determining obesity in a multifactorial mouse model. J Clin Invest. 1995 Apr;95(4):1545-52
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
02/23/2021
MGI 6.16
The Jackson Laboratory