Nqo1^tm1Akj
Targeted Allele Detail

Summary

• Symbol: Nqo1^tm1Akj
• Name: NAD(P)H dehydrogenase, quinone 1; targeted mutation 1, Anil K Jaiswal
• MGI ID: MGI:2388490
• Synonyms: NQO1 -
• Gene: Nqo1 Location: Chr8:108114857-108129838 bp, - strand Genetic Position: Chr8, 54.08 cM
• Alliance: Nqo1^tm1Akj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46669
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 6 and flanking non-coding sequences were replaced with a 2 kb neomycin resistance cassette via homologous recombination. The targeted mutation results in deletion of 101 C-terminal residues and disrupts enzyme function. Gene expression was absent in homozygous mutant animals in all tissues examined (lung, liver, kidney, colong, skeletal muscle) by Northern blot and Western blot. (J:46669)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nqo1 Mutation: 24 strains or lines available

References

• Original: J:46669 Radjendirane V, et al., Disruption of the DT diaphorase (NQO1) gene in mice leads to increased menadione toxicity. J Biol Chem. 1998 Mar 27;273(13):7382-9
• All: 26 reference(s)