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Ightm2Cgn
Targeted Allele Detail
Nomenclature
Symbol: Ightm2Cgn
Name: immunoglobulin heavy chain complex; targeted mutation 2, University of Cologne
MGI ID: MGI:2388486
Synonyms: B18, B1-8i, IghB1-8>, IgH, B1-8-HC, VHEmua
Gene: Igh  Location: unknown  Genetic Position: Chr12, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:79556
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion
 
Mutation detailsHomologous recombination and subsequent in vitro cre mediated excision resulted in the replacement of endogenous sequence encompassing a 3' Igh-D element (DQ52) and the Igh-J elements with a recombined antibody variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8). The B1-8 variable region was modified to contain a point mutation in codon 92, inactivating the heptameric recombination signal sequence. Flow cytometry verified cell surface expression of the mutant protein product. (J:79556)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igh Mutation:  44 strains or lines available
Notes
The targeted IgH allele was of the a allotype.
References
Original:  J:79556 Sonoda E, et al., B cell development under the condition of allelic inclusion. Immunity. 1997 Mar;6(3):225-33
All:  73 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory