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Tnctm1Msch
Targeted Allele Detail
Nomenclature
Symbol: Tnctm1Msch
Name: tenascin C; targeted mutation 1, Melitta Schachner
MGI ID: MGI:2388370
Synonyms: tn-C -/-
Gene: Tnc  Location: Chr4:63959785-64020725 bp, - strand  Genetic Position: Chr4, 34.06 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:78340
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExon 2 was replaced with a single loxP site by homologous recombination followed by in vitro cre mediated excision. The deleted region encoded the signal peptide and the tenascin assembly domain. While normal transcript was undetected in homozygous mutant mice, weak expression of a transcript in which exon 1 was spliced to exon 3 was identified by Northern blot and RT-PCR analyses. Sequence analysis showed that the aberrant splicing introduced a frameshift mutation. Quantitative immunoblot analysis indicated that protein expression was limited to less than 0.05% of the wild-type level. Immunhistochemical analysis supported this finding. (J:78340)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tnc Mutation:  57 strains or lines available
References
Original:  J:78340 Evers MR, et al., Impairment of L-type Ca2+ channel-dependent forms of hippocampal synaptic plasticity in mice deficient in the extracellular matrix glycoprotein tenascin-C. J Neurosci. 2002 Aug 15;22(16):7177-94
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory