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Ciitatm1Wrth
Targeted Allele Detail
Nomenclature
Symbol: Ciitatm1Wrth
Name: class II transactivator; targeted mutation 1, Walter Reith
MGI ID: MGI:2388316
Synonyms: CiitaIV-, pIV-
Gene: Ciita  Location: Chr16:10488178-10527564 bp, + strand  Genetic Position: Chr16, 5.65 cM, cytoband B1
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:71102
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Modified isoform(s))
Mutation:    Intragenic deletion
 
Mutation detailsThe gene was specifically inactivated in non-bone marrow antigen presenting cells by deleting the pIV promoter and its associated exon 4. A loxP site was introduced upstream of pIV and a floxed neo cassette was introduced downstream of exon 4. Transient expression of cre recombinase in correctly targeted ES cells removed the entire floxed region (800 bp) containing the pIV promoter and exon 4. Ablation of type IV mRNA in Ifng-stimulated MEFs (mouse embyronic fibroblasts) derived from homozygous mutant animals was confirmed by RNase protection assay. Gene expression from the pI and pIII promoters was not affected by the pIV deletion. (J:71102)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ciita Mutation:  15 strains or lines available
References
Original:  J:71102 Waldburger JM, et al., Selective abrogation of major histocompatibility complex class II expression on extrahematopoietic cells in mice lacking promoter IV of the class II transactivator gene. J Exp Med. 2001 Aug 20;194(4):393-406
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/17/2016
MGI 6.05
The Jackson Laboratory