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Mbd2tm1Bh
Targeted Allele Detail
Nomenclature
Symbol: Mbd2tm1Bh
Name: methyl-CpG binding domain protein 2; targeted mutation 1, Brian Hendrich
MGI ID: MGI:2388002
Synonyms: Mbd2-, Mbd2tm1.1Brd
Gene: Mbd2  Location: Chr18:70568189-70626131 bp, + strand  Genetic Position: Chr18, 44.54 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:68356
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExon 2 was deleted and replaced with a promoter-less beta-geo cassette via homologous recombination. Northern blot confirmed the absence of normal transcript in homozygous mutant animals. RT-PCR analysis using primers to exon 1 and exon 3 detected a faint mutant read-through product. Western blot analysis of spleen and liver nuclear extracts from homozygous animals detected a small mutant protein presumed to be the translation product from exon 1, and would lack any functional domains. (J:68356)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mbd2 Mutation:  17 strains or lines available
References
Original:  J:68356 Hendrich B, et al., Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development. Genes Dev. 2001 Mar 15;15(6):710-23
All:  18 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2020
MGI 6.15
The Jackson Laboratory