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Targeted Allele Detail
Symbol: Smarca2tm1Mya
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; targeted mutation 1, Moshe Yaniv
MGI ID: MGI:2387988
Synonyms: BRM-
Gene: Smarca2  Location: Chr19:26605050-26778322 bp, + strand  Genetic Position: Chr19, 21.17 cM, cytoband C1
Germline Transmission:  Earliest citation of germline transmission: J:51536
Parent Cell Line:  CK35 (ES Cell)
Strain of Origin:  129S2/SvPas
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe majority of the exon encoding amino acids 120-176 was replaced with a neomycin resistance cassette via homologous recombination. RT-PCR analysis of liver RNA from homozygous mutant animals revealed a faint truncated transcript resulting from an alternative splice product which skips the disrupted exon. Western blot analysis of brain nuclear extracts detected a shortened protein product expressed at less than 1% the amount of wild-type protein. Co-immunoprecipitation experiments showed that the mutant protein lacked functional capabilities. (J:51536)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 64 assay results
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Smarca2 Mutation:  53 strains or lines available
Original:  J:51536 Reyes JC, et al., Altered control of cellular proliferation in the absence of mammalian brahma (SNF2alpha). EMBO J. 1998 Dec 1;17(23):6979-91
All:  14 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.12
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