Hdac9^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Hdac9^tm1Eno
• Name: histone deacetylase 9; targeted mutation 1, Eric N Olson
• MGI ID: MGI:2387834
• Synonyms: HDAC9^-
• Gene: Hdac9 Location: Chr12:34097579-34967094 bp, - strand Genetic Position: Chr12, 15.06 cM, cytoband B2
• Alliance: Hdac9^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78734
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 4 and all of exon 5 were replaced by a cassette containing lacZ and neo inserted by homologous recombination. The deleted region encoded amino acids 99 through 176, encompassing the MEF2 binding domain, thus affecting the predominate MEF2-interacting transcriptional repressor (MITR) splice form. RT-PCR and sequence analysis of mutant mice identified aberrant transcript in which the inserted cassette was excised and exon 3 had spliced to exon 6. This alternate splice pattern introduced two stop codons immediately downstream of exon 3, and therefore precluded the translation of normal protein. (J:78734)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hdac9 Mutation: 49 strains or lines available

References

• Original: J:78734 Zhang CL, et al., Class II histone deacetylases act as signal-responsive repressors of cardiac hypertrophy. Cell. 2002 Aug 23;110(4):479-88
• All: 17 reference(s)