Sftpc^tm1Swg
Targeted Allele Detail

Nomenclature

• Symbol: Sftpc^tm1Swg
• Name: surfactant associated protein C; targeted mutation 1, Stephan W Glasser
• MGI ID: MGI:2387813
• Synonyms: SP-C(-)
• Gene: Sftpc Location: Chr14:70520941-70524081 bp, - strand Genetic Position: Chr14, 36.32 cM

Airspace remodeling, stromal thickening and monocytic infiltration in the lungs of Sftpc^tm1Swg/Sftpc^tm1Swg mice

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69619
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion of a PGK-neo cassette into exon 2. No mature mRNA was detected in homozygous mutant animals via Northern blot of lung tissue, but a faint band of smaller size was observed. RT-PCR and sequence analysis revealed the product was an alternatively spliced transcript from exon 1 to exon 3, bypassing exon 2. No protein product was detected in alveolar lavage from homozygous mutant animals. (J:69619)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sftpc Mutation: 8 strains or lines available

Notes

ES cell line = D3R.

References

• Original: J:69619 Glasser SW, et al., Altered stability of pulmonary surfactant in SP-C-deficient mice. Proc Natl Acad Sci U S A. 2001 May 22;98(11):6366-71
• All: 7 reference(s)