Tcf3^tm2Zhu
Targeted Allele Detail

Summary

• Symbol: Tcf3^tm2Zhu
• Name: transcription factor 3; targeted mutation 2, Yuan Zhuang
• MGI ID: MGI:2387351
• Synonyms: E47^bm
• Gene: Tcf3 Location: Chr10:80245348-80269481 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tcf3^tm2Zhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47671
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The DNA binding domain of the E47 isoform was disrupted by the introduction of two arginine to glycine missense mutations generated by site directed mutagenesis. While this mutation putatively disrupts DNA binding it was not expected to affect homodimerization of the E47 isoform. Semiquantitative RT-PCR analysis showed a 40% reduction of transcript produced from the targeted locus. The mutation was shown to affect splicing as the E47/E12 transcript ratio was determined to be increased by approximately 80% in homozygous mutant mice. (J:47671)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf3 Mutation: 39 strains or lines available

References

• Original: J:47671 Zhuang Y, et al., Functional replacement of the mouse E2A gene with a human HEB cDNA. Mol Cell Biol. 1998 Jun;18(6):3340-9
• All: 4 reference(s)