Brca2^tm1Mbn
Targeted Allele Detail

Summary

• Symbol: Brca2^tm1Mbn
• Name: breast cancer 2, early onset; targeted mutation 1, L Michelle Bennett
• MGI ID: MGI:2387337
• Synonyms: B2^-, Brca2 delta10/11
• Gene: Brca2 Location: Chr5:150446095-150493794 bp, + strand Genetic Position: Chr5, 89.52 cM
• Alliance: Brca2^tm1Mbn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74077
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Nucleotides 1745 to 2033, encompassing portions of exons 10 and 11 and the intervening intron, were replaced with a neomycin selection cassette. RT-PCR and sequence analyses identified an aberrant transcript containing the first 569 codons of the endogenous gene fused to 75 neo codons. This transcript was undetected by Northern blot analysis, indicating that it was unstable. (J:74077)

Disease models

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Brca2^tm1Mbn

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brca2 Mutation: 132 strains or lines available

References

• Original: J:74077 Bennett LM, et al., BRCA2-null embryonic survival is prolonged on the BALB/c genetic background. Mol Carcinog. 2000 Jul;28(3):174-83
• All: 3 reference(s)