Runx1^tm1Dez
Targeted Allele Detail

Summary

• Symbol: Runx1^tm1Dez
• Name: runt related transcription factor 1; targeted mutation 1, Dong-Er Zhang
• MGI ID: MGI:2387293
• Synonyms: AML1-ETO, Runx1^{tm1(CBFA2T1)Dez}
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm1Dez page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:38705
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A construct containing human exon 5 followed by the entire human CBFA2T1 coding region was inserted at the endogenous locus via homologous recombination, replacing the endogenous exon 5. The targeted allele generates a fusion protein similar to the fusion protein resulting from T(8;21) which is associated with the M2 subtype of acute myeloid leukemia (AML). Transcription of the allele was verified in heterozygous mice by a protection assay. (J:38705)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 35 strains or lines available

References

• Original: J:38705 Yergeau DA, et al., Embryonic lethality and impairment of haematopoiesis in mice heterozygous for an AML1-ETO fusion gene. Nat Genet. 1997 Mar;15(3):303-6
• All: 1 reference(s)