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Targeted Allele Detail
Symbol: Trp53tm1Glo
Name: transformation related protein 53; targeted mutation 1, Guillermina Lozano
MGI ID: MGI:2386273
Synonyms: p53-, p53R172Hdeltag
Gene: Trp53  Location: Chr11:69580359-69591873 bp, + strand  Genetic Position: Chr11, 42.83 cM, cytoband B2-C
Germline Transmission:  Earliest citation of germline transmission: J:61683
Parent Cell Line:  AB1 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprt+
Allele Type:    Targeted
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsA two-step procedure was employed to introduce an arginine to histidine substitute at residue 172 (R172H), the equivalent of R175H in humans. First, a neo-derived cassette replaced exons 2-6 by homologous recombination. Next, a vector containing exons 2-6 and carrying a CGC to CAC missense mutation at codon 172 was used to replace the neo cassette and reconstitute the gene. SSCP confirmed successful integration of the mutant allele and restriction digest analysis confirmed reconstitution of the gene. Immunoprecipitation experiments with mouse embryonic fibroblasts derived from homozygous mutant embryos showed that mutant protein is expressed at a level similar to wild-type. RT-PCR analysis detected full-length transcript and 2 mutant transcripts involving exon 3 deletions due to a mutation at the splice acceptor site of intron 2. (J:61683)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Trp53 Mutation:  141 strains or lines available
Original:  J:61683 Liu G, et al., High metastatic potential in mice inheriting a targeted p53 missense mutation. Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4174-9
All:  5 reference(s)

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