Trp53^tm1Glo
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Glo
• Name: transformation related protein 53; targeted mutation 1, Guillermina Lozano
• MGI ID: MGI:2386273
• Synonyms: p53^-, p53R172Hdeltag
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Glo page

The embryonic lethality of Cops6^{Gt(RRI087)Byg}/Cops6^{Gt(RRI087)Byg} mice can be partially rescued by concomitant loss of Trp53

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61683
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A two-step procedure was employed to introduce an arginine to histidine substitute at residue 172 (R172H), the equivalent of R175H in humans. First, a neo-derived cassette replaced exons 2-6 by homologous recombination. Next, a vector containing exons 2-6 and carrying a CGC to CAC missense mutation at codon 172 was used to replace the neo cassette and reconstitute the gene. SSCP confirmed successful integration of the mutant allele and restriction digest analysis confirmed reconstitution of the gene. Immunoprecipitation experiments with mouse embryonic fibroblasts derived from homozygous mutant embryos showed that mutant protein is expressed at a level similar to wild-type. RT-PCR analysis detected full-length transcript and 2 mutant transcripts involving exon 3 deletions due to a mutation at the splice acceptor site of intron 2. (J:61683)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm1Glo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 232 strains or lines available

References

• Original: J:61683 Liu G, et al., High metastatic potential in mice inheriting a targeted p53 missense mutation. Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4174-9
• All: 11 reference(s)