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Ikbkbtm1Ver
Targeted Allele Detail
Nomenclature
Symbol: Ikbkbtm1Ver
Name: inhibitor of kappaB kinase beta; targeted mutation 1, Inder M Verma
MGI ID: MGI:2386269
Synonyms: IKK2-
Gene: Ikbkb  Location: Chr8:22659212-22706589 bp, - strand  Genetic Position: Chr8, 11.42 cM, cytoband A3-A4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:54323
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsTwo exons encoding amino acids 36-106 of the kinase domain were replaced with a PGK-neo cassette via homologous recombination. The targeted mutation destroys lyine 44, a residue necessary for kinase activity. Homozygous mutant embryos were detected by PCR genotype analysis up to day E14.5. Sequence analysis of RT-PCR products from mouse embryonic fibroblasts (derived from E12.2 homozygous mutant embryos) revealed an in-frame deletion from nucleotides 106-318. However, Western blot analysis using antibodies directed against the C-terminus of Ikbkb failed to detect protein product. (J:54323)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ikbkb Mutation:  31 strains or lines available
References
Original:  J:54323 Li Q, et al., Severe liver degeneration in mice lacking the IkappaB kinase 2 gene [see comments]. Science. 1999 Apr 9;284(5412):321-5
All:  6 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/29/2016
MGI 6.06
The Jackson Laboratory