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Targeted Allele Detail
Symbol: Fli1tm1Dkw
Name: Friend leukemia integration 1; targeted mutation 1, Dennis K Watson
MGI ID: MGI:2386167
Gene: Fli1  Location: Chr9:32422204-32542861 bp, - strand  Genetic Position: Chr9, 17.74 cM
Germline Transmission:  Earliest citation of germline transmission: J:63242
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Hypomorph)
Mutation:    Insertion
Mutation detailsA floxed neo cassette was inserted into exon 9 via homologous recombination resulting in a premature stop codon between the DNA-binding domain and the CTA domain. The targeted mutation results in a hypomorphic allele. Northern blot analysis of homozygous mutant embryos showed 2 gene transcripts at 10% the level of wild-type transcription. Western blot analysis detected a truncated protein expressed at very low levels. Enzyme assay determined that the truncated protein exhibits 50%-60% of the activity of normal protein. (J:63242)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fli1 Mutation:  15 strains or lines available
TC1-10 ES cells were used.
Original:  J:63242 Spyropoulos DD, et al., Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol Cell Biol. 2000 Aug;20(15):5643-52
All:  16 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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