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Targeted Allele Detail
Symbol: Krt6a/Krt6btm1Cou
Name: keratin 6A; targeted mutation 1, Pierre A Coulombe
MGI ID: MGI:2385807
Synonyms: K6alpha/beta null
Gene: Krt6a  Location: Chr15:101689932-101694307 bp, - strand  Genetic Position: Chr15, 56.97 cM
Growth retardation and tongue blisters in Krt6a/Krt6btm1Cou/Krt6a/Krt6btm1Cou mice

Show the 3 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:64033
Parent Cell Line:  CK35 (ES Cell)
Strain of Origin:  129S2/SvPas
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intergenic deletion
Mutation detailsTwo closely linked genes were deleted using a single targeting event. The entire Krt2-6a gene, 13 kb of intergenic sequences, and the first half of Krt2-6b exon 1 were replaced with a neomycin resistance cassette via homologous recombination. RT-PCR and Northern blot analysis of cultured keratinocytes derived from homozygous mutant animals did not detect Krt2-6a or Krt2-6b transcripts, however a probe to the neo gene yielded positive results. Western blot analysis also failed to detect either Krt2-6a or Krt2-6b protein products. (J:64033)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Krt6a Mutation:  14 strains or lines available
Original:  J:64033 Wong P, et al., Introducing a null mutation in the mouse K6alpha and K6beta genes reveals their essential structural role in the oral mucosa. J Cell Biol. 2000 Aug 21;150(4):921-8
All:  2 reference(s)

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