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Targeted Allele Detail
Symbol: Hif1atm2Rsjo
Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 2, Randall S Johnson
MGI ID: MGI:2385361
Gene: Hif1a  Location: Chr12:73901375-73947530 bp, + strand  Genetic Position: Chr12, 31.99 cM
Germline Transmission:  Earliest citation of germline transmission: J:48084
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
Mutation detailsSequence encoding the basic helix-loop-helix domain was disrupted by the insertion of a construct containing lacZ followed by a floxed neo cassette via homologous recombination. The neo cassette was excised by the transient transfection of targeted ES cells by cre recombinase. Expression of lacZ was visualized via Xgal staining. (J:48084)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hif1a Mutation:  11 strains or lines available
Original:  J:48084 Ryan HE, et al., HIF-1 alpha is required for solid tumor formation and embryonic vascularization. EMBO J. 1998 Jun 1;17(11):3005-15
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
The Jackson Laboratory