Cxcl13^tm1Cys
Targeted Allele Detail

Summary

• Symbol: Cxcl13^tm1Cys
• Name: C-X-C motif chemokine ligand 13; targeted mutation 1, Jason G Cyster
• MGI ID: MGI:2384500
• Synonyms: BLC^-, CXCL13^-
• Gene: Cxcl13 Location: Chr5:96104810-96108927 bp, + strand Genetic Position: Chr5, 47.29 cM, cytoband E3
• Alliance: Cxcl13^tm1Cys page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78282
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Base pairs 18 through 116 (exon 2) were replaced by a cassette containing a stop codon followed by an internal ribosome entry site, an enhanced green fluorescent protein gene, and a floxed neo gene. The deleted region encoded amino acids 27 through 60, including 3 of the 4 conserved Cys residues. The absence of transcript and protein in homozygous mutant mice was confirmed by Northern and Western blot analyses of homozygous mutant mice. (J:78282)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cxcl13 Mutation: 19 strains or lines available

References

• Original: J:78282 Ansel KM, et al., A chemokine-driven positive feedback loop organizes lymphoid follicles. Nature. 2000 Jul 20;406(6793):309-14
• All: 30 reference(s)