Slc12a7^tm1Tjj
Targeted Allele Detail

Summary

• Symbol: Slc12a7^tm1Tjj
• Name: solute carrier family 12, member 7; targeted mutation 1, Thomas J Jentsch
• MGI ID: MGI:2383981
• Synonyms: Kcc4^-/-
• Gene: Slc12a7 Location: Chr13:73881213-73964873 bp, + strand Genetic Position: Chr13, 40.15 cM, cytoband C1
• Alliance: Slc12a7^tm1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76136
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Homologous recombination introduced a floxed neo cassette preceding the exon encoding transmembrane span 1 and part of transmembrane span 2 and a third loxP site was introduced after the exon encoding the remainder of transmembrane span 2. The floxed regions were deleted via transient expression of cre recombinase in correctly targeted ES cells. RT-PCR analysis detected a truncated transcript in mutant animals. Western blot analysis did not detect protein product in various tissues from homozygous mutant mice. (J:76136)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc12a7 Mutation: 67 strains or lines available

References

• Original: J:76136 Boettger T, et al., Deafness and renal tubular acidosis in mice lacking the K-Cl co-transporter Kcc4. Nature. 2002 Apr 25;416(6883):874-8
• All: 1 reference(s)