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Cd55tm1Mme
Targeted Allele Detail
Nomenclature
Symbol: Cd55tm1Mme
Name: CD55 molecule, decay accelerating factor for complement; targeted mutation 1, M Edward Medof
MGI ID: MGI:2383980
Synonyms: Cd55-, Daf1-
Gene: Cd55  Location: Chr1:130439027-130462744 bp, - strand  Genetic Position: Chr1, 56.89 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:72210
Parent Cell Line:  GK129 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsA loxP site was introduced with a neo-TK cassette into exon 3 and a second loxP site was introduced with a TK-Hph cassette into exon 5 via homologous recombination. Transient expression of cre recombinase in correctly targeted ES cells removed floxed regions containing exons 3-5 and the 2 TK genes. The deleted exons encode complement control protein (CCP) modules 2 and 3. RT-PCR analysis detected a truncated mRNA in homozygous mutant animals lacking exons 3-5. Functional assays confirmed the mutant protein was devoid of protein activity. (J:72210)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd55 Mutation:  25 strains or lines available
References
Original:  J:72210 Lin F, et al., Tissue distribution of products of the mouse decay-accelerating factor (DAF) genes. Exploitation of a Daf1 knock-out mouse and site-specific monoclonal antibodies. Immunology. 2001 Oct;104(2):215-25
All:  23 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/14/2017
MGI 6.08
The Jackson Laboratory