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Targeted Allele Detail
Symbol: Grk5tm1Rjl
Name: G protein-coupled receptor kinase 5; targeted mutation 1, Robert J Lefkowitz
MGI ID: MGI:2383968
Synonyms: grk5-, GRK5 KO, GRK5-KO
Gene: Grk5  Location: Chr19:60889749-61095968 bp, + strand  Genetic Position: Chr19, 56.52 cM
Germline Transmission:  Earliest citation of germline transmission: J:59179
Parent Cell Line:  AK7 (ES Cell)
Strain of Origin:  129S4/SvJaeSor
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsA loxP site was inserted upstream of exon 7 and a floxed TK-neo derived cassette was inserted downstream of exon 8 via homologous recombination. Exons 7 and 8 encode critical subelements I, II, and III of the protein kinase catalytical domain. The floxed region containing exons 7 and 8 and the TK-neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Western blot analysis of brain membranes from homozygous mutant animals confirmed the absence of gene expression. (J:59179)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Grk5 Mutation:  35 strains or lines available
Original:  J:59179 Gainetdinov RR, et al., Muscarinic supersensitivity and impaired receptor desensitization in G protein-coupled receptor kinase 5-deficient mice. Neuron. 1999 Dec;24(4):1029-36
All:  21 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory