Ube2b^tm1Jhjh
Targeted Allele Detail

Summary

• Symbol: Ube2b^tm1Jhjh
• Name: ubiquitin-conjugating enzyme E2B; targeted mutation 1, Jan H J Hoeijmakers
• MGI ID: MGI:2183933
• Synonyms: HR6b-, mHR6B-, Ube2b^-
• Gene: Ube2b Location: Chr11:51876324-51891589 bp, - strand Genetic Position: Chr11, 31.82 cM
• Alliance: Ube2b^tm1Jhjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35244
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 1 was disrupted by the insertion of a neomycin selection cassette immediately following the start codon. Northern blot analysis showed an absence of transcript in homozygous mutant mice. The absence of protein produced from Ube2b was determined via Western blot analysis of testicular extracts. Further analysis showed that the level of protein produced from the homologous Ube2a locus was unaffected by the targeting event. (J:35244)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube2b Mutation: 21 strains or lines available

References

• Original: J:35244 Roest HP, et al., Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification. Cell. 1996 Sep 6;86(5):799-810
• All: 11 reference(s)