Lamc1^tm1Umr
Targeted Allele Detail

Summary

• Symbol: Lamc1^tm1Umr
• Name: laminin, gamma 1; targeted mutation 1, Ulrike Mayer
• MGI ID: MGI:2183782
• Synonyms: laminin gamma^-
• Gene: Lamc1 Location: Chr1:153094668-153208532 bp, - strand Genetic Position: Chr1, 65.3 cM
• Alliance: Lamc1^tm1Umr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76368
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The genomic region encoding domain III, which contains the nidogen-binding molecule gamma1III4, was deleted and a floxed HSV-TK-neo cassette inserted into the downstream intron via homologous recombination. The HSV-TK-neo cassette was removed by transient expression of cre recombinase in correctly targeted cells. RT-PCR analysis verified the mutant allele in homozygous mutant ES cells. The deletion of gamma1III4 did not appear to interfere with the correct splicing of gamma1III3 to gamma1III5. Ablation of nidogen-binding activity was confirmed in homozygous mutant embryoid bodies via heparin affinity chromatography and rotary shadow electron microscopy. (J:76368)

Expression

In Structures Affected by this Mutation:

22 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lamc1 Mutation: 143 strains or lines available

References

• Original: J:76368 Willem M, et al., Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development. Development. 2002 Jun;129(11):2711-22
• All: 6 reference(s)