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Targeted Allele Detail
Symbol: Lamc1tm1Umr
Name: laminin, gamma 1; targeted mutation 1, Ulrike Mayer
MGI ID: MGI:2183782
Synonyms: laminin gamma-
Gene: Lamc1  Location: Chr1:153218922-153332786 bp, - strand  Genetic Position: Chr1, 65.3 cM
Germline Transmission:  Earliest citation of germline transmission: J:76368
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe genomic region encoding domain III, which contains the nidogen-binding molecule gamma1III4, was deleted and a floxed HSV-TK-neo cassette inserted into the downstream intron via homologous recombination. The HSV-TK-neo cassette was removed by transient expression of cre recombinase in correctly targeted cells. RT-PCR analysis verified the mutant allele in homozygous mutant ES cells. The deletion of gamma1III4 did not appear to interfere with the correct splicing of gamma1III3 to gamma1III5. Ablation of nidogen-binding activity was confirmed in homozygous mutant embryoid bodies via heparin affinity chromatography and rotary shadow electron microscopy. (J:76368)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 24 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Lamc1 Mutation:  114 strains or lines available
Original:  J:76368 Willem M, et al., Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development. Development. 2002 Jun;129(11):2711-22
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory