Itga2^tm1Ztr
Targeted Allele Detail

Summary

• Symbol: Itga2^tm1Ztr
• Name: integrin alpha 2; targeted mutation 1, Mary M Zutter
• MGI ID: MGI:2183401
• Synonyms: alpha2^-
• Gene: Itga2 Location: Chr13:114969617-115068636 bp, - strand Genetic Position: Chr13, 64.61 cM
• Alliance: Itga2^tm1Ztr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77798
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was flanked by an upstream floxed neo cassette and a loxP site inserted into intron 1. Transient transfection of ES cells with cre recombinase expression plasmids resulted in the excision of the neo cassette and exon 1. Flow cytometric analysis of platelets obtained from homozygous mutant mice showed an absence of surface expression. Western blot analysis of protein lysates from platelets and mammary glands confirmed the lack of protien. Neither full-length nor truncated transcript was detected by RT-PCR analysis of mammary gland lysates. (J:77798)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itga2 Mutation: 79 strains or lines available

References

• Original: J:77798 Chen J, et al., The alpha(2) integrin subunit-deficient mouse: a multifaceted phenotype including defects of branching morphogenesis and hemostasis. Am J Pathol. 2002 Jul;161(1):337-44
• All: 17 reference(s)