Fut2^tm1Sdo
Targeted Allele Detail

Summary

• Symbol: Fut2^tm1Sdo
• Name: fucosyltransferase 2; targeted mutation 1, Steven E Domino
• MGI ID: MGI:2183220
• Synonyms: FUT2^-, Fut2-LacZ
• Gene: Fut2 Location: Chr7:45298015-45315818 bp, - strand Genetic Position: Chr7, 29.41 cM
• Alliance: Fut2^tm1Sdo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72946
• Parent Cell Line: Pat5 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of the single coding exon of the gene was replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the catalytic domain. Homozygous mutant animals were identified by Southern blot and PCR genotype analysis. (J:72946)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fut2 Mutation: 22 strains or lines available

Notes

The embryonic stem cell lines Pat5 and R1 were used for gene targeting.

References

• Original: J:72946 Domino SE, et al., Deficiency of Reproductive Tract alpha(1,2)Fucosylated Glycans and Normal Fertility in Mice with Targeted Deletions of the FUT1 or FUT2 alpha(1,2)Fucosyltransferase Locus. Mol Cell Biol. 2001 Dec;21(24):8336-45
• All: 18 reference(s)