Gabra5^tm1.1Uru
Targeted Allele Detail

Summary

• Symbol: Gabra5^tm1.1Uru
• Name: gamma-aminobutyric acid type A receptor subunit alpha 5; targeted mutation 1.1, Uwe Rudolph
• MGI ID: MGI:2183170
• Synonyms: alpha5(H105R)
• Gene: Gabra5 Location: Chr7:57057420-57159807 bp, - strand Genetic Position: Chr7, 33.52 cM
• Alliance: Gabra5^tm1.1Uru page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77343
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: This allele was generated by breeding mice carrying Gabra5^tm1Uru with transgenic mice expressing cre recombinase via the EIIa promoter. The floxed neomycin cassette was excised from intron 3, while the point mutation in codon 105 that results in a histidine to arginine substitution remained intact. Western blot analysis showed that the expression of the encoded protein was decreased by approximately 20% in homozygous mutant mice. Autoradiographs showed novel diazepam-insensitve sites in regions where the endogenous protein is expressed, indicating the disruption of the drug-binding site. (J:77343)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabra5 Mutation: 30 strains or lines available

References

• Original: J:77343 Crestani F, et al., Trace fear conditioning involves hippocampal alpha5 GABA(A) receptors. Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8980-5
• All: 9 reference(s)