Runx1t1^tm1Fc
Targeted Allele Detail

Summary

• Symbol: Runx1t1^tm1Fc
• Name: RUNX1 translocation partner 1; targeted mutation 1, Franco Calabi
• MGI ID: MGI:2183012
• Synonyms: MTG8^Ex2/lacZ
• Gene: Runx1t1 Location: Chr4:13743436-13893649 bp, + strand Genetic Position: Chr4, 5.88 cM, cytoband A
• Alliance: Runx1t1^tm1Fc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70590
• Parent Cell Line: CCB (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The gene was disrupted by an in-frame insertion of a lacZ-neo cassette into the 3' end of exon 2, which is the common splice acceptor site for several upstream exons. RNase protection assay using a cDNA probe showed the absence the exon 2-4 splice product in brains of homozygous mutants, but an alternative splice product upstream of exon 3 was increased. Western blot of brain extracts from homozygous mutants showed an absence of the 75 kDa and 90 kDa products, presence of the 200 kDa product, and an increase in the 55 kDa product. (J:70590)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1t1 Mutation: 35 strains or lines available

References

• Original: J:70590 Calabi F, et al., Gene targeting reveals a crucial role for mtg8 in the gut. Mol Cell Biol. 2001 Aug;21(16):5658-66
• All: 3 reference(s)