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Targeted Allele Detail
Symbol: Slc22a1tm1Ahs
Name: solute carrier family 22 (organic cation transporter), member 1; targeted mutation 1, Alfred H Schinkel
MGI ID: MGI:2182947
Synonyms: Oct1-
Gene: Slc22a1  Location: Chr17:12867756-12894716 bp, - strand  Genetic Position: Chr17, 8.63 cM
Germline Transmission:  Earliest citation of germline transmission: J:70604
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExon 7 encoding putative transmembrane domains 7-9 and flanking intronic sequences were replaced with a PGK-hygro cassette in the reverse orientation via homologous recombination. Northern blot analysis did not detect gene expression in liver or kidney of homozygous mutant animals. However, RNase protection assay using a probe to nucleotides 447 to 691 lying outside the deleted region detected extremely low levels of transcript, which may represent a truncated and unstable message. Western blot analysis of homozygous mutant liver and kidney samples using a polyclonal antibody probe recognizing the C-terminal region of rat and mouse protein further verified the absence of gene expression. (J:70604)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Slc22a1 Mutation:  21 strains or lines available
Original:  J:70604 Jonker JW, et al., Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene. Mol Cell Biol. 2001 Aug;21(16):5471-7
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory