Per2^tm1Drw
Targeted Allele Detail

Summary

• Symbol: Per2^tm1Drw
• Name: period circadian clock 2; targeted mutation 1, David R Weaver
• MGI ID: MGI:2182832
• Synonyms: mPer2^ldc, Per2^-, Per2^m
• Gene: Per2 Location: Chr1:91343704-91387046 bp, - strand Genetic Position: Chr1, 46.13 cM, cytoband C5
• Alliance: Per2^tm1Drw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69626
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 and a portion of exon 6 were replaced with a neomycin selection cassette. Several transcript forms produced from the targeted allele were identified in homozygous mutant mice via RT-PCR and RACE analyses of brain RNA. The different transcripts involved aberrant splicing of exon 4 to a pseudoexon in intron 4, to a cryptic splice site within neo, and to exon 7. In spite of the transcripts having in-frame regions, normal protein was undetected in by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period. (J:69626)

Expression

In Mice Carrying this Mutation:	4 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Per2^tm1Drw

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Per2 Mutation: 71 strains or lines available

References

• Original: J:69626 Bae K, et al., Differential Functions of mPer1, mPer2, and mPer3 in the SCN Circadian Clock. Neuron. 2001 May;30(2):525-36
• All: 51 reference(s)