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Mark2tm1Hpw
Targeted Allele Detail
Nomenclature
Symbol: Mark2tm1Hpw
Name: MAP/microtubule affinity regulating kinase 2; targeted mutation 1, Helen Piwnica-Worms
MGI ID: MGI:2182819
Synonyms: Enk-, Mark2/EMK-, Par-1b-
Gene: Mark2  Location: Chr19:7275396-7341860 bp, - strand  Genetic Position: Chr19, 5.32 cM, cytoband B
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:68830
Parent Cell Line:  RW-4 (ES Cell)
Strain of Origin:  129X1/SvJ
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe insertion of a neomycin selection cassette deleted sequence from exons 2, 3, and 4. The replaced region encoded residues of the glycine-rich motif involved in MgATP-binding. Western blot analysis of extracts from fibroblasts showed an absence of all known isoforms produced from the targeted allele in homozygous mutant embryos. Immunoprecipitates of tissue extracts derived from homozygous mutant mice failed to phosphorylate GST-Cdc25C in vitro, incdicating ablation of kinase activity. (J:68830)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 15 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mark2 Mutation:  57 strains or lines available
References
Original:  J:68830 Hurov JB, et al., Immune system dysfunction and autoimmune disease in mice lacking Emk (Par-1) protein kinase. Mol Cell Biol. 2001 May;21(9):3206-19
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/30/2020
MGI 6.15
The Jackson Laboratory