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Transgene Detail
Symbol: Tg(CAG-cre/Esr1*)5Amc
Name: transgene insertion 5, Andrew P McMahon
MGI ID: MGI:2182767
Synonyms: CAG-CreERT2, CAGG-Cre, CaggCreER, CAGGCre-ER, CAGG-CreERT2, CAGGCre-ERTM, CAGGCre-ERtm line 5.8, CAGGS-CreER, CAGGS-CreErT, CMV-creERT, Cre-ER, Cre-ERTM, CreESRT, ER-cre, Tg(CAG-cre/Esr1)5Amc, TgCAGGCreER, TgCreER, Tg(cre/Esr1)5Amc, uCreERT
Transgene: Tg(CAG-cre/Esr1*)5Amc  Location: unknown  
Strain of Origin:  (C57BL/6 x CBA)F1
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    induced by tamoxifen
Mutation:    Insertion
Mutation detailsThis transgene expresses a fusion protein consisting of Cre recombinase joined to the ligand-binding domain of a mouse estrogen receptor modified to bind to 4-hydroxytamoxifen, but not to endogenous estrogen. The CAG promoter, containing a chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early (CMV-IE) enhancer, drives high levels of expression in most tissues. In the presence of tamoxifen, the fusion protein is transported into the nucleus, where cre can excise loxP-flanked segments from conditionally modified genes. (J:76130)
 Tissue activity of this recombinase allele
Driver: CAG     Summary of all recombinase alleles driven by CAG.

View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Homozygous transgenic mice are not viable or fertile. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

In transgenic mice the mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 fusion protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing loxP sites flanking a sequence of interest, tamoxifen induced, Cre-mediated targeted deletions are generated in the offspring. Tamoxifen administration will induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice.

Original:  J:76130 Hayashi S, et al., Efficient recombination in diverse tissues by a tamoxifen-inducible form of cre: a tool for temporally regulated gene activation/inactivation in the mouse. Dev Biol. 2002 Apr 15;244(2):305-18
All:  312 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.01
The Jackson Laboratory