Gne^tm1Rhk
Targeted Allele Detail

Summary

• Symbol: Gne^tm1Rhk
• Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase; targeted mutation 1, Rudiger Horstkorte
• MGI ID: MGI:2182685
• Gene: Gne Location: Chr4:44034075-44084177 bp, - strand Genetic Position: Chr4, 23.1 cM, cytoband B1
• Alliance: Gne^tm1Rhk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76298
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was replaced with a neomycin resistance gene cassette via homologous recombination. Homozygous mutant embryos were observed at a frequency of 10% and 6% at E8.5 and E9.5, respectively, by PCR genotype analysis. Because homozygous mutant animals could not be obtained, embryonic stem cell lines from E3.5 homozygous mutant embryos were established. RT-CR and Western blot analysis of the mutant stem cell lines confirmed the absence of gene expression. (J:76298)

Expression

In Mice Carrying this Mutation:

52018 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gne Mutation: 47 strains or lines available

References

• Original: J:76298 Schwarzkopf M, et al., Sialylation is essential for early development in mice. Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5267-70
• All: 4 reference(s)