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Targeted Allele Detail
Symbol: Itgb1tm1Ehi
Name: integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Emilio Hirsch
MGI ID: MGI:2182640
Synonyms: Beta(1de6)
Gene: Itgb1  Location: Chr8:128685654-128733200 bp, + strand  Genetic Position: Chr8, 76.09 cM, cytoband E2
Developmental defects in Itgb1tm1Ehi/Itgb1tm1Ehi embryos

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:76216
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Inserted expressed sequence, Null/knockout)
Mutation:    Insertion
Mutation detailsA duplication of exon 6 (an exon common to all known splice variants) was inserted into intron 6 along with stop codons, a polyadenylation signal, and a neo gene. A frameshift arises from the splicing of exon 6 to its duplicate, resulting in the substitution of the isoform-specific carboxy terminal sequences with a new carboxy terminal sequence encoding 11 residues. RT-PCR, immunoprecipitation, and immunolabeling analyses showed that the mutant isoform was the only isoform produced and that the altered carboxy terminal region did not affect localization of the protein in homozygous mutant mice. (J:76216)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 8 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Itgb1 Mutation:  13 strains or lines available
Original:  J:76216 Hirsch E, et al., Defective Rac-mediated proliferation and survival after targeted mutation of the beta1 integrin cytodomain. J Cell Biol. 2002 Apr 29;157(3):481-92
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
The Jackson Laboratory