Cyp1a1^tm1.1Dwn
Targeted Allele Detail

Summary

• Symbol: Cyp1a1^tm1.1Dwn
• Name: cytochrome P450, family 1, subfamily a, polypeptide 1; targeted mutation 1.1, Daniel W Nebert
• MGI ID: MGI:2182307
• Synonyms: Cyp1a1(-)
• Gene: Cyp1a1 Location: Chr9:57595211-57611107 bp, + strand Genetic Position: Chr9, 31.34 cM
• Alliance: Cyp1a1^tm1.1Dwn page

Dioxin-induced hepatic injury in Cyp1a1^tm1.1Dwn/Cyp1a1^tm1.1Dwn and Cyp1a2^tm1Dwn/Cyp1a2^tm1Dwn mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59398
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre recombinase RNA was injected into zygotes heterozygous for Cyp1a1^tm1Dwn. A region containing a floxed HPRT minigene and the entire coding region of Cyp1a1 was excised by cre recombinase. Northern and Western blot analyses of total RNA and protein extracts obtained from animals treated with TCDD (to enhance expression levels) showed an absence of endogenous transcript and normal protein in the livers of homozygous mutant mice. (J:59398)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp1a1 Mutation: 38 strains or lines available

References

• Original: J:59398 Dalton TP, et al., Targeted knockout of Cyp1a1 gene does not alter hepatic constitutive expression of other genes in the mouse [Ah] battery. Biochem Biophys Res Commun. 2000 Jan 7;267(1):184-9
• All: 29 reference(s)