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Transgene Detail
Symbol: Tg(Col2a1-PTHLH)1Ecw
Name: transgene insertion 1, Eleanor C Weir
MGI ID: MGI:2182284
Synonyms: col II-PTHrP
Transgene: Tg(Col2a1-PTHLH)1Ecw  Location: unknown  
Subgross analysis of skeletons of Tg(Col2a1-PTHLH)1Ecw/0 mice

Show the 2 phenotype image(s) involving this allele.

Strain of Origin:  (C57BL/6 x SJL)F2
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(Col2a1-PTHLH)1Ecw expresses 1 gene
Mutation detailsA 568 bp fragment of human PTHLH cDNA under the control of the mouse Col2a1 promoter was used as the transgene. The Col2a1 promoter sequences were used to target expressioin of the transgene to proliferating and prehypertrophic chondrocytes. An artificial exon was engineered into the 5' end of the Col2a1 construct to prevent alternative splicing and the translation initiation codon was inactivated to ensure translation of the human PTHLH gene. 2.2 kb of human growth hormone untranslated sequences were added downstream of PTHLH to provide termination and polyadenylation signals as well as increase expression efficiency. Transgenic animals express human PTHLH at approximately 4x the amount of endogenous mouse protein. (J:76983)
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Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:76983 Weir EC, et al., Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation. Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10240-5
All:  3 reference(s)

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