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Isl1tm1Tmj
Targeted Allele Detail
Nomenclature
Symbol: Isl1tm1Tmj
Name: ISL1 transcription factor, LIM/homeodomain; targeted mutation 1, Thomas M Jessell
MGI ID: MGI:2182246
Synonyms: Isl1delta
Gene: Isl1  Location: Chr13:116298281-116309689 bp, - strand  Genetic Position: Chr13, 64.87 cM, cytoband D2.3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:31131
Parent Cell Line:  W9.5/W95 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neomycin selection cassette was inserted such that the exon encoding the second LIM domain was deleted and that splicing from the exon encoding the first LIM domain to the exon encoding the homeodomain would result in a frameshift mutation. In situ hybridization did not detect transcript produced from this allele in the neural tube of homzygous mutant mice, but message was detected in the mesoderm ventral to the neural tube. Immunohistochemical analysis of homozygous mutant embryonic sections showed a lack of protein in all analyzed tissues. (J:31131)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 80 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Isl1 Mutation:  16 strains or lines available
References
Original:  J:31131 Pfaff SL, et al., Requirement for LIM homeobox gene Isl1 in motor neuron generation reveals a motor neuron-dependent step in interneuron differentiation. Cell. 1996 Jan 26;84(2):309-20
All:  10 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/13/2016
MGI 6.05
The Jackson Laboratory