Pcsk6^tm1Rob
Targeted Allele Detail

Summary

• Symbol: Pcsk6^tm1Rob
• Name: proprotein convertase subtilisin/kexin type 6; targeted mutation 1, Elizabeth J Robertson
• MGI ID: MGI:2181754
• Synonyms: pace4^-, spc4-
• Gene: Pcsk6 Location: Chr7:65511884-65700134 bp, + strand Genetic Position: Chr7, 35.36 cM
• Alliance: Pcsk6^tm1Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62181
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 280 bp of the gene were replaced with a neomycin resistance cassette via homologous recombination. The deleted region encodes a catalytic serine essential for enzymatic activity. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot analysis of total brain mRNA using an antisense RNA probe encompassing the targeted region. (J:62181)

Expression

In Structures Affected by this Mutation:

16 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pcsk6^tm1Rob

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcsk6 Mutation: 94 strains or lines available

References

• Original: J:62181 Constam DB, et al., SPC4/PACE4 regulates a TGFbeta signaling network during axis formation. Genes Dev. 2000 May 1;14(9):1146-55
• All: 9 reference(s)