Kif3b^tm1Noh
Targeted Allele Detail

Summary

• Symbol: Kif3b^tm1Noh
• Name: kinesin family member 3B; targeted mutation 1, Nobukata Hirakawa
• MGI ID: MGI:2181744
• Synonyms: kif3B^-
• Gene: Kif3b Location: Chr2:153133333-153175310 bp, + strand Genetic Position: Chr2, 75.41 cM, cytoband G-H2
• Alliance: Kif3b^tm1Noh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51419
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of most of exon 1 with a neomycin resistance cassette via homologous recombination resulting in the deletion of sequences encoding the kinesin motor domain. Lack of gene expression in homozygous mutant E9.5 embryos was confirmed by Western blot analysis. (J:51419)

Disease models

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kif3b Mutation: 32 strains or lines available

References

• Original: J:51419 Nonaka S, et al., Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein. Cell. 1998 Dec 11;95(6):829-37
• All: 5 reference(s)