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Tgm2tm1Gml
Targeted Allele Detail
Nomenclature
Symbol: Tgm2tm1Gml
Name: transglutaminase 2, C polypeptide; targeted mutation 1, Gerry Melino
MGI ID: MGI:2180638
Synonyms: Tg2-, TG2-, TG2KO, Tgase2-
Gene: Tgm2  Location: Chr2:158116402-158146436 bp, - strand  Genetic Position: Chr2, 78.72 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:67573
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA 1.2 kb region of the gene spanning part of exon 5, intron 5, exon 6, and part of exon 6 was replaced with a neo resistance cassette via homologous recombination thus deleting the active site of exon 6 and results in the loss of protein cross-linking activity. Western blot of liver, thymus, brain, and erythrocyte samples and RT-PCR analysis of thymus from homozygous mutant animals verified the absence of gene expression. However, residual enzyme activity was detected in some tissues which the authors speculate may be attributed to Tgase1. (J:67573)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Tgm2 Mutation:  7 strains or lines available
References
Original:  J:67573 De Laurenzi V, et al., Gene disruption of tissue transglutaminase. Mol Cell Biol. 2001 Jan;21(1):148-55
All:  32 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory