Bglap/Bglap2^tm1Kry
Targeted Allele Detail

Summary

• Symbol: Bglap/Bglap2^tm1Kry
• Name: bone gamma carboxyglutamate protein; targeted mutation 1, Gerard Karsenty
• MGI ID: MGI:2180470
• Synonyms: Bglap^tm1Kry, Ocn^-, osc^ml
• Gene: Bglap Location: Chr3:88290808-88291771 bp, - strand Genetic Position: Chr3, 38.82 cM
• Alliance: Bglap/Bglap2^tm1Kry page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34440
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A single mutational event was used to target two genes, Bglap1 and Bglap2. A construct containing the neomycin resistance gene deleted exon 4 of Bglap1 and the entire coding region of Bglap2 by homologous recombination. A third locus in this cluster, Bglap-rs1, was left intact. Expression analysis of total RNA isolated from homozygous mutant bone showed an absence of normal transcript, while Western blot analysis revealed a lack of protein. A radioimmuno assay further confirmed the abrogation of the two loci. (J:34440)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bglap Mutation: 13 strains or lines available

References

• Original: J:34440 Ducy P, et al., Increased bone formation in osteocalcin-deficient mice. Nature. 1996 Aug 1;382(6590):448-52
• All: 24 reference(s)