Sgcd^tm1Mcn
Targeted Allele Detail

Summary

• Symbol: Sgcd^tm1Mcn
• Name: sarcoglycan, delta (dystrophin-associated glycoprotein); targeted mutation 1, Elizabeth M McNally
• MGI ID: MGI:2180193
• Synonyms: dsg^-, Scgd^-
• Gene: Sgcd Location: Chr11:46861904-47880309 bp, - strand Genetic Position: Chr11, 28.09 cM, cytoband B1.2
• Alliance: Sgcd^tm1Mcn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76730
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette was used to replace exon 2, which encodes the translation initiation codon and the cytoplasmic and transmembrane domains. Northern analysis showed that message was not transcribed in skeletal muscle of homozygous mutant mice. The lack of protein in immunoblots further confirmed complete inactivation. (J:76730)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sgcd Mutation: 22 strains or lines available

References

• Original: J:76730 Hack AA, et al., Differential requirement for individual sarcoglycans and dystrophin in the assembly and function of the dystrophin-glycoprotein complex. J Cell Sci. 2000 Jul;113(Pt 14):2535-44
• All: 43 reference(s)