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Mapkapk2tm1Mgl
Targeted Allele Detail
Nomenclature
Symbol: Mapkapk2tm1Mgl
Name: MAP kinase-activated protein kinase 2; targeted mutation 1, Matthias Gaestel
MGI ID: MGI:2179724
Synonyms: MK2-, MK2 KO
Gene: Mapkapk2  Location: Chr1:131053700-131097826 bp, - strand  Genetic Position: Chr1, 56.89 cM, cytoband E4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:59678
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe catalytic domain of this gene, encompassing subdomains V and VI, was replaced with a neomycin resistance cassette via homologous recombination which introduced stop codons in all 3 reading frames and results in a truncated kinase lacking amino acids 130-383. Northern blot of spleen from homozygous mutant animals verified the absence of gene expression and kinase assay of mouse embryonic fibroblasts verified the absence of enzyme activity. (J:59678)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mapkapk2 Mutation:  20 strains or lines available
References
Original:  J:59678 Kotlyarov A, et al., MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis [see comments]. Nat Cell Biol. 1999 Jun;1(2):94-7
All:  44 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory