About   Help   FAQ
Mapkapk2tm1Mgl
Targeted Allele Detail
Nomenclature
Symbol: Mapkapk2tm1Mgl
Name: MAP kinase-activated protein kinase 2; targeted mutation 1, Matthias Gaestel
MGI ID: MGI:2179724
Synonyms: MK2-, MK2 KO
Gene: Mapkapk2  Location: Chr1:131053700-131097826 bp, - strand  Genetic Position: Chr1, 56.89 cM, cytoband E4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:59678
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe catalytic domain of this gene, encompassing subdomains V and VI, was replaced with a neomycin resistance cassette via homologous recombination which introduced stop codons in all 3 reading frames and results in a truncated kinase lacking amino acids 130-383. Northern blot of spleen from homozygous mutant animals verified the absence of gene expression and kinase assay of mouse embryonic fibroblasts verified the absence of enzyme activity. (J:59678)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mapkapk2 Mutation:  22 strains or lines available
References
Original:  J:59678 Kotlyarov A, et al., MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis [see comments]. Nat Cell Biol. 1999 Jun;1(2):94-7
All:  47 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
06/13/2017
MGI 6.10
The Jackson Laboratory